Effects of one-step aging, two-step aging of low-temperature and high-temperature and heating rate of aging on the microstructure and mechanical properties of Ti-10V-2Fe-3Al titanium alloy after high temperature deformation were studied. The results show that comparing with one-step aging, in the two-step aging the early precipitated ω particles provided uniform nucleation sites for α phase during the low-temperature aging. As a result, the two-step aging sequences led to much finer and more uniform α+β microstructure to obtain good match of strength and ductility. For a slow heating rate of 0.1 oC/s to the peak aging temperature, a very finely dispersed isothermal ω-phase had enough time to precipitate, and thus provided nucleation sites of high density for the α-phase, resulting in the very fine α plates; therefore, the same match effect of strength and ductility was obtained