On the basis of using sodium citrate as a reducing agent to reduce chloroauric acid, a synthesis-controlled method of gold nanoparticles is presented by using the pulsed micromixing technology actuated by PZT actuators. The synthesis mechanism of gold nanoparticles and working principle of pulsed micromixing are analysed. According to different concentration ratio (Na3C6H5O7:HAuCl4) and pulsed mixing frequency, the relevant controlled synthesis tests of gold nanoparticles are designed and carried out. The characteristics of obtained specimen including optical properties, particle size, particle deviation and monodispersity are characterized using UV-vis and TEM. The effect of concentration ratio and frequency to the test results is analyzed. The test results show that controlled synthesis of gold nanoparticles can be realized using the pulsed micromixing method based on piezoelectric actuation, by controlling the concentration ratio of two preparations and pulsed mixing frequency.