In this paper, low surface energy film and a cylindrical micro structure was prepared on titanium alloy by plasma vapor deposition and femtosecond laser technology, the surface morphology of samples were characterized by using scanning electron microscope, the hydrophobicity of samples can be evaluated by using titration angle method, at the same time, the mechanical properties of the film was also tested , the best process was obtained. Finally composting the above two kinds of technologies, the bionic super-hydrophobic surface was prepared on the titanium alloy , the results showed that the surface of titanium alloy with excellent super hydrophobic properties can be obtained by using the composite method of films and microstructures. The water contact angle of the column microstructure with Cu film can reach 156°and its rolling angle can reach 8°.