Tensile behaviors of a Nickel-based single crystal superalloy DD13 were studied from room temperature to 1050 °C in this study. Results show that both of yield strength and tension strength reached the peak value, namely 1108 MPa and 1340 MPa when testing temperature reached 700 °C. However, a converse trend was observed for the temperature dependence of plastic properties. Model of dislocation overcoming the precipitation strengthening phase (γ′) takes a key role on yield strength, which changes from shearing to climbing with the rising testing temperature. After the yield point, both of tension strength and plastic property are decided by that whether dislocations could slide smoothly or not. Relational twisting of partial dislocations with stacking fault from different slipping systems was observed at 700 °C.