Quasicrystal is a new material, there are great research value and application potential in its special structure and property. The Al63Cu25Fe12 quasi-crystal-alloy was produced by vacuum non-consumable arc smelting copper mold casting technology, and then the Al63Cu25Fe12 melt-spinning with different cooling rates was prepared by vacuum single roll spin quenching technology. The effect of different cooling rates on the phase structure and micro-structure of the alloy was researched through using various analytical ways, such as X-ray Diffraction (XRD), Differential Scanning Calorimeter (DSC), Optical Microscope (OM), Scanning Electron Microscope (SEM), Energy Dispersive Spectrometer (EDS), Transmission Electron Microscope (TEM) and others. The result shows that the cooling rate of copper mold-casting was about 1×102Ks, and the corresponding cold speed of the ribbon strip prepared under different speeds (500/2000/4000) r/min is 6.37×105K/s, 1.77×106K/s, 3.98 ×106K/s. Copper-mold-casting samples contained λ phase (AL13Fe), quasi-crystal I phase (Al63Cu25Fe12), β phase (ALFe(Cu)) and τ phase (AlCu(Fe)). The quasicrystal I phase was mainly distributed around λ phase. When the cooling rate of melt-spinning reaches (105-106)K/s, the phase structure consists of quasicrystalline I phase with icosahedron structure and β phase with a little Cscl simple cubic structure.