Biomedical materials have excellent mechanical properties, stability and biocompatibility, and are widely used in dentistry, orthopedics, medical devices and other fields. However, various infection problems are obviously occurred in the current use of biomedical materials, which are severely harmful human health and life safety. Micro-arc oxidation technology can construct antibacterial film on the surface of biomedical metal materials such as titanium and magnesium, which is one of the surface modification technologies to effectively solve the infection problem. In this paper, the research status and mechanism of particle incorporation into micro-arc oxide film are summarized. On this basis, the latest development of antibacterial surface constructed by the incorporation of Ag, Ag2O, Cu, CuO, ZnO and other metal particles into micro-arc oxidation film in recent years was reviewed. The effects of particle incorporation on corrosion resistance and wear resistance of micro-arc oxidation film are described. Meanwhile, the application and development of micro-arc oxidation antibacterial film with particle incorporation are prospected.