In this work, the influence of warm rolling deformation degree on the microstructure evolution and high temperature (760 ℃) mechanical properties of a novel Ni-based superalloy were investigated by EBSD, SEM, TEM and quasi-static high temperature tensile tests. The results show that the reduction of warm rolling can significantly influence the high temperature mechanical properties. The echancement of yield strength, ultimate tensile strength and elongation for high temperature mechanical properties of Ni-based superalloy processed by the warm rolling with the deformation reduction of 10%, compared to that of samples processed by stardard heat treatments, are 230 MPa, 166 MPa, and 4.1%, respectively. Further rolling deformation processing (80%) causes the evident increase (22.5%) of elongation, generating the excellent strength-ductility synergy effect. The results indicate that the increase of strength and ductility can attribute to the transformation of the doformnation mechanism when the tensile test is conducted at 760 ℃. The transition from stacking fault to micro twinning can obtain the high temperature tensile strength-ductility synergy.