Creep loading has a certain effect on the service performance of structural components. In this paper, the creep preloading on Ti6321 titanium alloy with equiaxed, bimodal and Widmanstatten microstructures were carried out. Dynamic compression tests of the material after creep investigated at a strain rate about 3000s-1. The dynamic behavior of Ti6321 titanium alloy after different creep stress loading was studied. The results show that the yield stress decreases and the compressive strength increases after creep preloading. With the increase of creep stress, the impact absorption energy of titanium alloy with three microstructures after dynamic compression increases and the adiabatic shear sensitivity decreases. Titanium alloys without creep have phase transition bands after dynamic compression, Titanium alloy with equiaxed microstructure and bimodal microstructure after dynamic compression after creep preloading do not fail, only produce deformation band. Titanium alloy with Widmanstatten microstructure fails due to phase transition bands. Adiabatic shear of equiaxed and bimodal microstructure are delayed by creep.