In this study, porous Ti-6Al-4V alloy was prepared by diffusion bonding method using Ti6Al4V mesh. The microstructure, tensile and fatigue properties were studied after various heat treatment methods, i.e., 780℃/2h, FC; 950℃/2h, FC; 950℃/2h, FC + 540/4h, FC. The results showed that the elastic modulus and tensile strength of porous Ti-6Al-4V alloy, can be adjusted in the range of 9.5 ~ 12.2GPa and 360 ~ 505MPa, respectively. The fatigue strength can reach 40 ~ 80MPa at R = -1, f = 10Hz and N = 5′10₆ under cyclic loading conditions. In addition, it was found that the heat treatments have a greater effect on tensile strength than for elastic modulus.