The cube textured Ni-7at%W(Ni7W) substrates were prepared starting from spark plasma sintered ingot, followed by Rolling-Assisted Biaxially Textured Substrate (RABiTS) processing. According to the EBSD and XRD analyses of the fully annealed tape, the cube grain content of the tape reaches as high as 99.4% within a tolerance angle of less than 10o and the grain boundary length content is 93.6% within a tolerance angle of less than 10o, while the FWHM values of the in-plane and out-of-plane orientations are about 6.32o by (111) Phi-scan and 5.4o by (200) ω-scan, respectively, indicating that the tapes have sharp cube texture. The Ni7W substrate has a uniform grain size and high quality of cube texture. The La2Zr2O7 (LZO) buffer layer fabricated on Ni7W substrates shows that the epitaxially textured LZO buffer layer could be formed on the as-obtained Ni7W substrate. The FWHM values of (222) φ-scan and (400) ω-scan are 7.57° and 5.73°, respectively