Mo-Cu alloys with different Fe content were prepared by an infiltration method. The microstructure and distribution of Fe of the alloys were analyzed by field-emission scanning electron microscope (FESEM) and energy disperse spectroscopy (EDS). The influence of Fe content on the density, thermal conductivity, electrical conductivity, hardness and microstructure was investigated. The results show that 1% Fe addition (mass fraction) increases the density and the hardness of the Mo-Cu alloys, but decreases thermal conductivity and electrical conductivity; meanwhile the microstructure becomes more compact, and Cu distributes in the form of mesh, and Mo skeleton sintering necks become bigger. However, too much Fe is not conducive to the improvement of properties