郭丽,朱晶心,贾兰,柯靖,吉立静.丝素蛋白-金纳米粒子复合体系的稳定性研究[J].稀有金属材料与工程,2016,45(1):217~221.[Guo Li,Zhu Jingxin,Jia Lan,Ke Jing,Ji Lijing.The Stability of Silk Fibroin-Gold Nanoparticles Composite System[J].Rare Metal Materials and Engineering,2016,45(1):217~221.]
丝素蛋白-金纳米粒子复合体系的稳定性研究
投稿时间:2013-12-30  修订日期:2014-05-14
中文关键词:  丝素蛋白  金纳米粒子  稳定性
基金项目:国家自然科学基金,山西省青年科技研究基金,山西省自然科学基金
中文摘要:
      柠檬酸钠还原法制备的金纳米粒子与再生丝素蛋白复合得到了高度稳定的丝素蛋白-金纳米粒子(SF-Au NPs)体系。研究了SF-Au NPs在不同pH值以及KCl浓度的磷酸盐缓冲液中的稳定性,应用紫外-可见光光谱(UV-Vis)、透射电子显微镜(TEM)和红外光谱(FTIR)等测试方法对SF-Au NPs体系进行了表征。结果表明:SF-Au NPs在3~11的pH范围内及50~1000 mM的KCl溶液中均具有良好的稳定性。以柠檬酸钠和牛血清白蛋白稳定的金纳米粒子作为对照实验,结果表明SF-Au NPs的稳定性主要与丝素蛋白空间阻隔效应有关。本文报道的制备方法简单、高效、成本低,SF-Au NPs体系有望在生物医学领域显示出广阔的应用前景。
The Stability of Silk Fibroin-Gold Nanoparticles Composite System
英文关键词:silk fibroin  gold nanoparticles  stability
英文摘要:
      In this study, highly stable SF-Au NPs were obtained using regenerated protein silk fibroin (SF) to stabilize gold nanoparticles (Au NPs), which were prepared through citrate reduction method. The stability of SF-Au NPs in buffer solutions with different pH conditions and different KCl concentrations were systematically investigated. Ultraviolet-visible spectroscopy (UV-Vis)、 transmission electron microscopy (TEM) and fourier transform infrared spectroscopy (FTIR) were employed to characterize the SF-Au NPs system. The results revealed that SF afforded the Au NPs with remarkable stability in the pH range of 3 to 11 and salt concentrations range of 50 mM to 1000 mM. Taking citrate and BSA stabilized gold nanoparticles as controls, the results indicated that steric hindrance may play an dominated role for the stability of SF-Au NPs. The proposed method was simpler, more efficient, and more cost effective than the conventional modification strategies for Au NPs; thus, SF–Au NPs can be potentially used in biomedical applications.
作者单位E-mail
郭丽 太原理工大学 guoli0169@link.tyut.edu.cn 
朱晶心 太原理工大学 zhujingxin@tyut.edu.cn 
贾兰 太原理工大学  
柯靖 太原理工大学  
吉立静 太原理工大学  
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