In the present study, Isothermal compression tests were performed to investigate the hot deformation behavior of TA1/AZ31B multi-layer composites. Flow stress-strain curves were obtained for deformation at temperature from 573K to 723K with the strain rate range from 0.01s-1 to 10s-1, and the height reduction range from 30% to 50%. Effect of the strain on the flow stress, both TA1 and AZ31B had plastic deformation during the compression bonding process. However, all layers deformation of TA1/AZ31B multi-layer composite is not occur at the same time and not uniform. The deformation of AZ31B is larger than that of TA1, and AZ31B occurs dynamic recrystallization during the deformation process. When strain rate is 10s-1, the middle TA1 layer occurs necking and fracture. Based on the flow stress-strain curves, the Arrhenius-type constitutive equation could precisely predict the flow stress behavior of TA1/AZ31B multi-layer composite. Besides, the value of the correlation coefficient was 0.991 when that of average absolute relative error was 3.976%. The processing map at true strain of 0.5 for TA1/AZ31B multi-layer composite was developed using the dynamic materials model (DMM) theory. The processing map of isothermally compressed TA1/AZ31B multi-layer composite exhibited that the optimum parameters were determined at the temperature of 723K and the strain rate of 0.01s-1 with power dissipation’s maximum efficiency of 28%. In the meanwhile, there was one instability domain in the processing map of isothermally compressed TA1/AZ31B multi-layer composite, it is in the deformation temperature range from 573K to 692K and the strain rate range from 0.6s?1 to 10 s?1.