丁祥龙,李少冰,郭泽鸿,卢海宾,赖春花,吴静漪,高岩,王敬旭,徐淑兰.表面电荷的调控对钛表面纤维连接蛋白吸附能力影响的研究[J].稀有金属材料与工程,2020,49(7):2431~2435.[Ding Xianglong,Li Shaobing,Guo Zehong,Lu Haibin,Lai Chunhua,Wu Jinyi,Gao Yan,Wang Jingxu,Xu Shulan.Regulation of surface charge on titanium surfaces for fibronectin adsorption[J].Rare Metal Materials and Engineering,2020,49(7):2431~2435.]
表面电荷的调控对钛表面纤维连接蛋白吸附能力影响的研究
投稿时间:2019-06-28  修订日期:2019-11-21
中文关键词:  表面电荷  蛋白  种植体  细胞
基金项目:国家自然科学基金资助(项目号81600900);南方医科大学培育基金(项目号 PY2018016);南方医科大学口腔医院培育基金(项目号 PY2018N089)
中文摘要:
      目的 通过对钛片表面电荷的调控改变纯钛表面的蛋白吸附能力,改善其生物学性能。方法 在钛片表面制作大颗粒喷砂酸蚀的形貌,并分别浸泡在不同的溶液中。分别观察检测不同样本表面形貌及表面元素构成;各组样本的表面粗糙度;以及各组样本表面的电荷状况;最后检测各组样本的纤维连接蛋白吸附能力。结果 各组样本表面具有相似的表面形貌,不同组表面因为浸泡的液体不同其表面元素各不相同;各组样本的表面粗糙度无显著差异;而蛋白吸附能力方面浸泡在氯化钙溶液中的钛片具有最高蛋白吸附能力(P<0.01),而对照组大颗粒喷砂酸蚀组吸附能力最弱。结论 将钛片浸泡在含有二价钙离子的氯化钙溶液中可以改变钛片表面的电荷状态,提升蛋白吸附能力,从而改善其生物学性能。
Regulation of surface charge on titanium surfaces for fibronectin adsorption
英文关键词:Surface charge  protein  implant  cell
英文摘要:
      Objective The purpose of this study was to investigate the fibronectin adsorptive capacity of pure titanium surfaces and improve their biological properties by regulating their surface charge. Methods The different surfaces of titanium sheet were obtained by sandblasting with large grit and acid- etching (SLA), and then immersing in different solutions. Surface morphology, elemental composition, roughness, and urface charge status were studied, and fibronectin adsorption assay was performed for comparative analysis. Result The surface morphology of each group was similar, but the surface elements of the different groups were different because of immersion in different liquids. There was no significant difference in the surface roughness of each group. Titanium sheets immersed in calcium chloride solution had the highest protein adsorption capacity (P < 0.01), whereas the control group had the lowest adsorption capacity. Conclusions Immersin. Of titanium sheets in calcium chloride solution containing divalent calcium ions can change their surface charge, enhance their protein adsorption capacity, and improve their biological properties.
作者单位E-mail
丁祥龙 南方医科大学口腔医院种植中心 dingxianglong88@126.com 
李少冰 南方医科大学口腔医院种植中心  
郭泽鸿 南方医科大学口腔医院种植中心  
卢海宾 南方医科大学口腔医院种植中心  
赖春花 南方医科大学口腔医院种植中心  
吴静漪 南方医科大学口腔医院种植中心  
高岩 南方医科大学口腔医院种植中心  
王敬旭 广州医科大学第一附属医院口腔科  
徐淑兰 南方医科大学口腔医院种植中心  
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