Commercial pure titanium (CP-Ti) sheets were cold rolled to different amounts, then annealed, and then re-rolled by 20%. Microstructure changes were investigated via electron backscatter diffraction (EBSD). After re-rolling, {112} <23> contraction twins and {102}<101> extension twins proliferate. A twin lamellas structure can be observed, caused by tangle of deformation twins and the generation of secondary and tertiary twins. There is no simple correlation between the average grain size and the amount of twinning. The grain size significantly decreases with the increase of pre-deformation degree, for 0.5 h annealed sample. Re-rolling tends to re-orient the lattice closer to ND. While the texture change and twin volume fraction are small and the {100}//RD fiber remains.