The amorphous carbon coating was synthesized on the TA2 substrate surface by direct current balanced magnetron sputtering. The corrosion resistance and conductivity of TA2 substrate and amorphous carbon coating were analyzed by electrochemical and surface contact resistance tests in proton exchange membrane fuel cell (PEMFC) with different F^- contents. The results show that the corrosion resistance and conductivity of amorphous carbon-coated TA2 alloy are better than those of bare TA2 substrate. When the F^- content is increased from 1×10^-6 mol/L to 1×10^-3 mol/L, the defect density of TA2 substrate and amorphous carbon coating is increased, indicating the decreased corrosion resistance of both materials. The amorphous carbon coating at potential of 0.6 V demonstrates the corrosion current density of 0.68 μA/cm² in the F^- environment with high content (1×10^-3 mol/L), still providing a good protection for TA2 substrate. Moreover, the amorphous carbon coating can also improve the surface conductivity of the TA2 substrate: the interface contact resistance decreases from 76.40 mΩ·cm² (TA2 substrate) to 6.52 mΩ·cm².