Single-phase V-based alloy membranes have potential applications in the field of hydrogen separation purification due to their higher hydrogen permeability and lower cost than Pd alloy membranes. The effect of Cr content on the hydrogen solubility and diffusivity, hydrogen permeability and hydrogen embrittlement resistance of single-phase V90-xTi10Crx(x=0,5,10,20) alloys has been investigated in this paper. The results show that the increase of Cr content decreases the hydrogen dissolution and hydrogen diffusion in V90-xTi10Crxalloy, thus decreasing the hydrogen permeability, but V90-xTi10Crx alloy still shows better hydrogen permeation performance than Pd and Pd-Ag alloys. Moreover, the fracture temperature of the V90-xTi10Crx alloy decreases from 184°C (x=5at%) to 141°C (x=10at%) with increasing Cr content, while x=20at% the membrane maintaines its integrity when air-cooling to room temperature, exhibiting excellent resistance to hydrogen embrittlement.