Laser powder-bed fusion (L-PBF) technology processes unique advantages in building complex-shape alloys. This paper invesgated the microstructure and high temperature low-cycle fatigue behavior of L-PBFed GH3536 superalloy in 855℃, and revealed the influence of heat treatment on microstructure and fatigue performance. By combining with different fatigue life prediction models, the fatigue damage behavior of L-PBFed GH3536 superalloy in various strain amplitudes was further discussed. These results are important for high temperature low-cycle fatigue life prediction and application of additive manufactured samples.