Mechanical alloying combined with spark plasma sintering was adopted to prepare W23.5Ta23.5Cr23.5V23.5Ti6 high entropy alloys. The effects of ball milling times on the elemental distribution and micrsstructure of the alloys were explored. It is found that the powder after 40 h of ball milling has a homogeneous particle size with about 3.65 ± 1.91 μm, and presents an equiaxed particle morphology. A dense uniform alloy can be obtained after the powder sintered by SPS at 1500 ℃. The relative density is above 99.0%. The WTaCrVTix solid solution with BCC structure is continuously distributed, in which the atomic ratios of W, Ta, Cr and V are close to the equiatomic ratios. A small amount of laves phase is distributed in the matrix. The TiO particles with FCC structure have an average size of 1.08±0.38 μm and are uniformly distributed in the matrix. Its room-temperature compressive yield strength, high-temperature compressive yield strength and microhardness are 2870 MPa、1954 MPa and 873.4 ± 7.6 HV, respectively.