To enhance the mechanical properties of Mo alloys crafted through laser powder bed fusion (LPBF), a hot isostatic pressing (HIP) treatment was utilized. Following HIP treatment, the porosity decreases from 0.27% to 0.22%, enabling the Ti elements to diffuse fully and distribute more uniformly within the Mo matrix, and forming a substantial number of low-angle grain boundaries (LAGBs). The tensile strength soared from 286 ± 32 MPa to 598 ± 22 MPa, while the elongation improved from 0.08 ± 0.02% to 0.18 ± 0.02%, without notable alterations in grain morphology during the tensile deformation. HIP treatment eliminated the molten pool boundaries (MPBs), which are the primary source of premature failure in LPBFed Mo alloys. Consequently, HIP treatment emerges as a novel and effective approach for strengthening the mechanical properties of LPBFed Mo alloys, offering a fresh perspective on crafting high-performance Mo-based alloys.