The addition of yttrium (Y) significantly enhances the properties of GH3535 alloy, making the alloy better applied in the molten salt reactors. However, the alloying elements are prone to segregation during the solidification process of alloy casting, which can significantly impair the service performance of the alloy. In this study, the influence of homogenization parameters on element segregation, dendritic structure and the precipitates evolution in the GH3535-0.08 wt.% Y alloy were observed by scanning electron microscopy, synchrotron radiation diffraction and so on. Additionally, specific homogenization parameters were maintained constant throughout the experiments. Results indicate that heat treatment at 1150 °C for 10 hours represents the optimal homogenization condition among those evaluated. Following this optimal treatment, dendrite structures and element segregation are completely eliminated. Furthermore, both SiC and Y5Si3 precipitates present in the as-cast alloy decrease significantly. Conversely, the homogenization at 1188 °C induces overheating defects within the alloy; SiC and Y5Si3 also decrease, but some large M6C phases are observed, which adversely affect subsequent forging processes.