Ti6321 alloy wire with a diameter of Ф2.9mm was produced by hole die drawing process, and the evolution of microstructure and mechanical properties in the forming process of Ti6321 alloy wire was studied in the paper. The results show that the average grain size of primary α phase decreases from 10.5μm to about 2.1μm, and the tensile strength increased from 1035MPa to 1250MPa. The tensile fracture surface with a typical ductile fracture of the wire was observed by scanning electron microscope,. And the texture in the wire was analyzed by EBSD, and found that there are (0001) ⊥ axial texture and {0001} <10-10> texture. It can be concluded that grain refinement and texture are the main cause for wire reinforcement.