The multipass hot rolling simulation of Ti-6Al-4V alloy was carried out by thermo-mechanical coupling method. The distribution characteristics of temperature and equivalent plastic strain at different passes on the cross section were studied. The simulation results showed that the temperature of surface was lower than that of the center. With increasing the rolling passes, the temperature of surface area decreased, the temperature of central increased first and then decreased. Equivalent plastic strains in both surface and center increased with increasing the rolling passes. The equivalent strain in the center was larger than that in the surface. The experimental results showed that with increasing the rolling passes, the deformation of microstructure in the central region was larger than that in the surface region. The temperature in the central region was higher and dynamic spheroidization ocurred easier.