PPy/rhBMP-2 composite coating on the pure Ti-substrate(Ti/PPy/rhBMP-2)has been successfully electrosynthesized by constant current method in aqueous media.The cyclic voltammetry demonstrated the redox reaction of the composite coating.125I radiolabeling technique was used to measure the amount of rhBMP-2 that was doped into Ti/PPy/rhBMP-2.Goniometer and SEM were used to characterize surface morphology.The effects of Ti/PPy/rhBMP-2 on the proliferation and differentiation of BMSCs were evaluated.The results indicated that rhBMP-2 could be incorporated into Ti/PPy/rhBMP-2 in the stable form of polyelectrolyte anion and the coating showed high roughness and loose structure.The doped rhBMP-2 can still keep the ability of osteoinduction and promote the proliferation and differentiation of BMSCs.