Ni microgrid transparent electrodes with different pore sizes (1.2，2.4，3.4，4.5 and 5.6 μm), were prepared via sputtering Ni into the gaps of PS colloidal crystal templates by magnetron sputtering. The influence of the pore sizes on the transmittance and the electric properties of the Ni microgrid transparent electrodes were investigated by SEM, UV-Vis spectrometer and four-point probe. It was found that the transmittance decreased and electric properties increased with the increase of the pore sizes of the samples. Ni microgrid transparent electrode showed a low electrical resistance and high transmittance in the ultraviolet-visible range. The results of theoretical calculation and AFM analysis indicated that the height of microgrid increased with the increase of the diameter of PS particles, which is the fundamental reason for the decrease of the transmission properties and the increase of electric properties of Ni microgrid transparent electrodes.