The titanium metals subjected to surface modification have good biocompatibility, bioactivity and low cell toxicity. It could induce apatite formation in vitro and bone bonding in vivo. However, the infection of implant is a serious problem for the clinic, so it is important to study the interaction of titanium metal with germs involving in the infection. The titanium metals subjected to anodic oxidation treatment (AO-Ti), acid-alkali treatment (AA-Ti) and alkali-heat treatment (AH-Ti) were used in this study. The germs of Gram-positive strain of Staphylococcus aureus (ATCC 25923) and Gram-negative strain of Escherichia coli（ATCC 25922）were cultured on these bioactive metals. The material surfaces were characterized by SEM and DSA. The effects of surface properties on the germs were evaluated by the investigation of the cell extrinsic shape, the proliferation of bacteria according to SEM and MTT observations. The results show that the Ti metals subjected to alkali-heat treatment (AH-Ti) and anodic oxidation treatment (AO-Ti) have good bacterial restrain properties. The titanium surface topography has great effect on the bacterial adhesion and proliferation on its surface