Glass fiber reinforced chitosan-hydroxyapatite (HA-CS) composites (G(f)/HA-CS) were prepared by a in-situ precipitation process. The infulences of the cross linker, HA mass fraction and fiber mass fraction on the structure and flexural strength of the composites were investigated. The structures and properties of the as-prepared composites were characterized by IR, SEM and universal test machine. The results show that the composites with good performances can be obtained by employing the in-situ hybrid process; the toughness of the composites modified by glutaric dialdehyde cross-linking can be remarkably increased, and their flexural strength can be improved by 16% to an optimum value of 84.47 MPa when the monomer ratio (CS/HA) reaches 10/1 and the fiber mass fraction reaches 1.5%. With the increase of glass fiber mass fraction, the microstructure of fractured surfaces changes from smooth to crapy. It can be infered that the toughness of the composites is improved