Diamond-like multilayer films were deposited on Ti6Al4V alloy substrate by filtered cathodic vacuum arc (FCVA) technique. The morphology of the films was observed with an atomic force microscope and the hardness was determined with a nano-indenter. Tribological properties and corrosion resistance of the diamond-like multilayer film in simulated body fluid were investigated using a micro-abrasion tribometer and an electrochemical testing system with three electrodes. Results show that the multilayer films consist of compact nanoparticles, and the surface roughness is about 4.86 nm; the hardness and elastic modulus of multilayer films are approximately 54.82 GPa and 342.27 GPa, respectively. In simulated body fluid, the diamond-like multilayer film remarkably improves the anti-wear ability and friction-reduction performances of the Ti6Al4V alloy substrate, and the wear rate of the multilayer film is only 11.7%-22.6% of Ti6Al4V alloy’s. With an increase of load, the corrosion potential of the film decreases and the corrosion current density increases; the deposited diamond-like multilayer films can effectively improve the corrosion resistance of Ti6Al4V alloy.