The nanoparticles dispersed in the liquid are influenced by the factors of surface wetting, inter-particle energy, double-layer electrostatic energy, space resistance function, etc. According to this theory, the best dispersion conditions for Ag nanoparticles in aqueous media were obtained through a method of high-speed shearing and ultrasound action by use of ABS, Tween 80 and sodium silicate compound, i.e. the concentration of sodium silicate, ABS and Tween 80 is 0.5%, 0.5% and 2% (mass fraction), respectively, and the ultrasonic time is 1.5 h. TEM observation reveals that nano-silver disperser was homogeneous and stable. Through the agar dilution method, the minimum inhibitory concentration (MIC) of nano-silver dispersion for the E. coli8099 and Staphylococcus aureus ATCC6538 are both 50 μg/g