The influencing rule of alloy elements (Mo, Zr, Nb), processing and heat treatment on the microstructure and mechanical properties of a near β type biomedical titanium alloy TiZrMoNb (TLE) were investigated systematically; the influential factors on biomechanical compatibility matching of the TLE alloy and how to keep it were discussed. The results show that increasing the contents of Zr and Mo can strengthen the alloy, whereas increasing the contents of Mo and Nb and larger hot working rate are helpful to decrease the elastic modulus. The hot-rolled alloy after solid solution treatment possesses lower strength and elastic modulus; while for the above-mentioned alloy with another aging treatment, the strength and elastic modulus are increased, due to dispersive strengthening and fine grain strengthening. The prepared alloy possesses better cold and hot wrought properties without obvious anisotropy, and higher strength, lower elastic modulus and good comprehensive matching of ductility and toughness and fatigue resistance can be obtained after adequate heat treatment