By means of the creep properties measurement and microstructure observation of the isothermally forged GH4169 alloy and the hot continuous rolled (HCR) alloy, the influence of the deformation technology on the microstructure and creep behavior of GH4169 alloy was investigated. Results show that the deformation features of the alloy during hot continuous rolling are identified as the twinning and dislocation slipping within the twins. Compared with the isothermally forged alloy, the denser dislocations formed during hot continuous rolling possess have an effect of deformed strengthening, which can improve the creep resistance of the alloy. Only the twinning deformation occurs in the forged alloy during creep, but the deformation mechanism of HCRed alloy during creep is thought to be the twinning and dislocation slipping within the twins. Thereinto, the denser dislocations formed during HCR of the alloy may promote the single/multi-systems slipping of creep dislocation activated within the twins, which may weaken the stress concentration to restrain or delay the initiation of the cracks in the grain boundaries. It is thought to be a main reason of prolonging the creep lifetime of the alloy. In the later period of creep, the micro-cracks are formed in the grain boundary regions vertical to the applied stress axis, and propagate along the grain boundary to produce the cleavage fracture, which is a main mechanism of creep fracture for the alloys prepared by the two kinds of technology.