The effect of preoxidation temperature on high temperature (1100 °C) cyclic oxidation resistance of GH3128 superalloy was studied by thermogravimetry combined with SEM, XRD and other analysis approaches. The results show that the high temperature cyclic oxidation resistance of GH3128 superalloy can be effectively improved by preoxidation at the appropriate temperature. The high temperature cyclic oxidation resistance of GH3128 superalloy after pre-oxidization depends on the combined effect of two factors. The first one is that the alloy element Cr in the surface of the alloys oxidizes selectively to form the protective oxide film, which is dense and uniform, to enhance the high temperature cyclic oxidation resistance of the alloy. The second one is that the alloy elements Ti and Cr segregate at grain boundaries in the surface of the alloys to accelerate the oxidation of grain boundary and to produce microscopic cracks near the boundary, and then reduce the high temperature cyclic oxidation resistance of the alloy. The samples pre-oxidized at 900 °C possess the best cyclic oxidation resistance due to that the protective oxide film is uniformly formed and the negative effect caused by the grain boundary segregation of Ti and Cr is relatively weak at 900 °C.