The pure titanium plates were modified by SAM using the organic solution with 3-aminopropyltriethoxysilane as the chief component and subsequently exposed to simulated body fluid for biomimetic mineralization. The surface morphologies and structures were characterized by attenuated total refraction-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electronic microscopy (SEM) and X-ray diffraction (XRD); Human sweat gland cells (HMSCs) were planted on the surfaces of samples before and after modification, and then the adhesion and proliferation of HMSCs on the surfaces of titanium and its action before and after modification were investigated by MTT colorimetry and SEM. The results show that HCA coating is synthesized by biomimetic method and the ratio of Ca/P is 1.3. Compared with the pure titanium samples, the adhered HMSCs on the surfaces of titanium after modification by SAM are more, and the proliferation effect is obvious.