The Re coating was deposited on C/C substrates by double-glow plasma technique. The microstructure, phases, Vickers hardness and interfacial bonding strength of the coating were studied. The results show that the Re coating is composed of poly-crystalline column structural grains with preferential growth orientation of (110) and (103) crystal plane. The Re coating is dense and smooth, and the size of grains ranges from 0.5 μm to 1.5 μm. The Vickers hardness of the Re coating is 6.66±0.25 GPa. The Re coating and C/C substrates inter-diffuses with each other during preparation and there is mechanical bonding and physical bonding in the interface. During the loading process of the scratch tests, the interfacial bonding strength between Re coating and C/C substrates is high even when the C/C substrate is broken due to the low hardness and ductility.