Copper and cerium were metallized into 304 stainless steel by the double glow plasma technique combined with the copper hollow cathode effect. Microstructure of the samples was investigated by metallographic microscope, scanning electron microscopy, energy spectrum analyzer and X-ray diffraction. The reasons why diffusion layers are divided into white layer and dark layer, of the segregation of carbon and chromium to the surface layer and the distribution variation of other elements were explained. Antibacterial experiments show that 304 stainless steel after infiltration has good antibacterial property to E. coli and S. aureus.