Ni(OH)2 nanoplate arrays grown on Ti substrate were synthesized via a hydrothermal method in solution containing nickel. The as-prepared samples were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The electrochemical properties of Ni(OH)2/Ti in NaOH solution were studied. The results show that the electrode exhibits good electrocatalytic activity toward the oxidation of glucose. The linear range for the determination of gluose is 4.5×10-5~1.05×10-3 mol/L with a detection limit of 5 μmol/L (S/N = 3)