The hydroxyapatite(HA)/Chitosan (CS) coating was prepared by a microarc oxidation process on the surface of the biomedical used titanium. The morphology and the phase composition of the coating were analyzed by using scanning electron microscope (SEM), X-ray diffractometer (XRD) and infrared spectrometer (FTIR). The toxicity of the coating material to the osteoblasts and its bioactivity in vivo were evaluated. The characteristics of the HA/CS coating by the microarc oxidation method, and its possibility of application in the bone regeneration were analyzed. The results show that the HA/CS coating on the surface of titanium with a thickness of 40±2 μm, a roughness of 3.3±0.1 μm, a porosity of (30±2)%, approximately circle pores and their diameter in the range of a few microns to 20 μm, and a bond strength of 20.5±1.1 MPa, can be prepared by using the microarc oxidation method. The HA and CS in the coating do not form chemical bonds. HA crystals occupy 10% of the total particles with an average particle size 37 nm. The other 90% are amorphous HA particles. Titanium base HA/CS coating can promote the proliferation of osteoblasts and it is not toxic. In addition, it can induce the formation of bone trabecula and shows a high bioactivity. In general, the HA/CS coating material with a high bioactivity will have a good application prospect in the aspect of bone regeneration