The samples of TC18 titanium alloy were produced by electron beam rapid manufacturing(EBRM). The effect of different wire feeding modes on microstructure and tensile properties of TC18 by EBRM were studied. The typical macrostructure of TC18 exhibit large columnar β grains nucleated at the substrate after EBRM, and the size of single wire is finer than that of double wire. However, both processes leads to flake primary α phase, β transformation microstructure and grain boundary α phase. While the amount of primary α phase of single wire is larger than that of double wire, the interlamellar spacing of single wire is smaller than double wire. The plasticity and stability of single wire are higher, and the strength is slightly lower than the double wire.