The non-magnetic Cu60Ni40 alloy substrates containing strong cube texture were prepared by rolling assisted biaxially textured technology (RABiTS). The surface textures of the Cu60Ni40 alloy substrates after cold-rolling and the recrystallization treatment were studied. The results show that the key factors that influence grain orientation in Cu60Ni40 alloy substrate are the total amount of rolling deformation and recrystallization annealing process. Through the large deformation of cold-rolling, the strong copper-type rolling texture in the Cu60Ni40 alloy substrates can be obtained. The content of the cube texture (≤10°), the fraction of small angle and Σ3 grain boundaries in the Cu60Ni40 alloy substrates prepared by optimized cold rolling and two-step recrystallization annealing process are 99.7%, 95.1% and 0.1%, respectively.