There’s no dispute that the control of interaction between cell and implant which includes drug delivery system and biosensor is a long-purchased goal. Recently, scientists are looking forward to exploring new materials in restricting cell adhesion and activity. In this essay, titanate tanowire was formed on the surface of titanium disks by hydrothermal route and the interaction between titanate tanowire and MG63 osteoblasts(including cell adhesion, proliferation and differentiation)was investigated. It’s shown that fewer cells were adhered on the smooth titanium disks rather than the nanowire one. Only a few cells were fixed on the tanowire surface which showed an irregular long spindle shape observed by SEM. Immunofluoresense also illustrated an irregular cell shape without forming a cell structure, the proliferation was restricted at the same time. Meanwhile, alkaline phosphatase and RUNX2 activity test shared the same results. It can be well concluded that cell adhesion and activity was seriously restricted titanate nanowire surface, such results can be applied on the further studies of drug delivery system and biosensor.