Through rolling assisted biaxially textured substrate route, X-ray diffraction and electron backscatter diffraction technique were used to research systematically the laws of orientation and texture formation in Ni-W alloy substrates with 5at.%, 7at.% and 9.3at.% of W contents during cold rolling deformation and recrystallizing heat-treatment processes. It finds that, during cold rolling deformation process, the increments of S and Copper orientation fractions present a downward trend, while that in Brass orientation fraction shows a rising trend with the increase of W content. Therefore, the alloy substrates with lower and higher W contents will gain Copper and Brass types rolling texture, respectively. During recrystallizion heat-treatment process, the cube nuclei in lower W content alloy substrates will initiate early and grow quickly, and then it will obtain cube texture easily by annexing other orientations. However, cube-oriented grains will nucleate and grow together with other orientation grains in higher W content alloy substrates. It will obtain random orientations finally because the growth rate of cube grains is slower than others.