The powder deformation behavior, microstructure evolution and mechanical properties of the Nb-35Ti-6Al-5Cr-8V-5C alloys after different ball milling time were investigated using powder metallurgy method by the combination of mechanical alloying and hot press sintering. The result show that the massive metal particles of Nb-35Ti-6Al-5Cr-8V-5C mixed powder are translated into flakes morphology firstly and then deformed into flocculent morphology during the action of impact extrusion as the ball milling time increasing, as well as the TiC powders are distributed uniformly on the surface of flake metal powder. The Nb-35Ti-6Al-5Cr-8V-5C alloy is consisted of Nbss phase and (Nb, Ti)C phase, and the volume of carbide for each alloy is about 11%. The element of Ti is mainly distributed in (Nb, Ti)C and the grain boundary of Nbss, however the element of Al, Cr, V is mainly distributed in Nbss grain. The size of Nbss and (Nb, Ti)C phase is decreased with the increasing of ball milling time. The compressive mechanical properties and plastic deformation capacity at room temperature is significantly increased causing by grain refinement of Nbss and reinforced phase dispersion of carbides. After compression deformation Nbss and carbide have good interfacial bonding, however, the obvious crack with a nearly parallel distribution inside carbides is existed. Furthermore, it is evident that the mechanical property of Nb-35Ti-6Al-5Cr-8V-5C alloy prepared by powder metallurgy is better than that prepared by arc melting method.