Tungsten-copper films were prepared on slide glass by magnetron sputtering. The structural analyses show that deposition rate and silver content increase, while grain size and microhardness of the films decreases with the copper target current. Solid solution is formed for the WCu films, and Cu particles are segregated at high Cu content. WCu films are highly hydrophobic, and film wettability, droplet size and ambient temperature/humidity affect the drying time of water droplet and water film. The antibacterial tests by plate counting and spraying of bacterial suspension show that WCu films have good antibacterial activity against E. coli. The WCu films are expected to be used in antimicrobial surface modification of environmental facilities.