The microstructure evolution of TC25G titanium alloy bar with basket microstructure after different deformation degrees were studied and the changes of its thermal stability and creep resistance at 550 ℃ were compared. The results show that the tensile plasticity after thermal exposure increases and the creep resistance decreases with the increase of deformation degree. The two mechanical properties match well at 100% deformation degree and can meet the requirements of engineering application. The increase of deformation degree corresponds to the spheroidizing process of lamellar α phase. Before the lamellar α phase is fully spheroidized, the interface strengthening effect of multi-layer structure makes the alloy have good creep resistance at high temperature. However, after α-phase spheroidization, the alloy has better plasticity because of the microstructure dominated by equiaxed microstructure. With the increase of deformation degree, the size of the fracture dimples becomes smaller and more uniform, and the dimple depth increases, which indicates that the plasticity after heat exposure increases . The results of nano-hardness test show that the microhardness of primary α phase is higher than that of β transforms, and the creep resistance can be improved by adjusting the content and distribution of α phase in the alloy by solution temperature, in order to obtain the best matching of strength and plasticity at high temperature, the extent of lamellar α phase spheroidization can be controlled.