With the increasing pollution of nitrogen oxides, the development of denitration catalysts have become one key factor for the treatment. In this paper, Ni0.09Ti0.91O2 nanotube supported copper denitration catalysts were prepared by hydrothermal and calcination two-step methods. The structure and catalytic denitration performance were studied. Results showed that the nanotube structure of the catalysts were determined by N2 adsorption-desorption, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and other methodologies. Ni0.09Ti0.91O2 nanotubes are anatase structures, copper atomic clusters are distributed on the surface of the nanotubes, and nitrogen adsorption and desorption tests determined that the specific surface area of Ni0.09Ti0.91O2 before and after loading copper was 263.51 and 216.5422 m2 g-1，respectively. The copper on the surface of the nanotubes was uniformly dispersed. The denitration efficiency of Ni0.09Ti0.91O2 nanotube with 7wt% copper was quite approach to 100%, which was higher than that of the nanotube catalyst without copper, and it had good anti-poisoning performance. The results of in-situ infrared spectroscopy diffuse reflection tests showed that the NH3-SCR process of Ni0.09Ti0.91O2-loaded copper followed the L-H mechanism. In this paper, the denitration performance of Ni0.09Ti0.91O2 nanoparticle-supported copper was also studied as a comparison. The catalyst of Ni0.09Ti0.91O2 nanotube loaded with 7wt% copper showed the best denitrification and anti-poisoning properties, which had good application prospects.