To enhance the mechanical properties of Mo alloys prepared through laser powder bed fusion (LPBF), a hot isostatic pressing (HIP) treatment was used. Results show that following HIP treatment, the porosity decreases from 0.27% to 0.22%, enabling the elements Mo and Ti to diffuse fully and to distribute more uniformly, and to forming a substantial number of low-angle grain boundaries. The tensile strength soars from 286±32 MPa to 598±22 MPa, while the elongation increases from 0.08%±0.02% to 0.18%±0.02%, without notable alterations in grain morphology during the tensile deformation. HIP treatment eliminates the molten pool boundaries, which are the primary source for premature failure in LPBFed Mo alloys. Consequently, HIP treatment emerges as a novel and effective approach for strengthening the mechanical properties of LPBFed Mo alloys, offering a fresh perspective on producing high-performance Mo-based alloys.