Low cyclic fatigue behavior of Ti-1023 titanium alloy has been investigated under a fully reversed total strain-controlled mode. The strain-life and cyclic stress-strain data were analyzed to determine the individual strain fatigue parameters. It is found that the Ti-1023 alloy exhibited an initial slight cyclic hardening, followed by stable cycle at a total strain amplitude of 0.6%-0.8%, then cyclic softening at a total strain amplitude of above 0.8% to 3.0%. It is considered that the cyclic stress response behavior of Ti-1023 alloy depends on the total strain amplitude.