The effect of duplex aging on low cycle fatigue behavior of Ti-10V-2Fe-3Al alloys was investigated. The cyclic stress response curves show that the cyclic softening occurs at the high strain amplitude of 1.5%, 1.2% and 1.0%. However, when the strain amplitude decreases to 0.8% and 0.6%, cyclic stress saturation appears. Low cycle fatigue life of duplex aging is similar to that of simplex aging. The relationship between cycle total strain amplitude and fatigue life fits to classic Coffin-Manson equation. The fractography analyses suggest that transgranular fracture with well-developed fatigue striations is the predominant failure mode. The plastic deformation traces and secondary cracks are also observed. TEM examination reveals that fatigue deformed microstructures are homogeneous for both duplex aging and simplex aging. Due to shortening operated time and saving energy, duplex aging is more beneficial to actual industrial production than simplex aging.