Plasma surface copper and nickel alloying was applied to Ti6Al4V alloy. The effects of treatment temperatures on the microstructures were investigated by scanning electron microscope (SEM). The chemical composition and phase constituents of the alloyed layer were analyzed using glow discharge spectrum (GDS) and X-ray diffraction (XRD). The results show that the alloyed layer prepared at 850 oC with about 7 μm in thickness is bonded strongly to the Ti6Al4V substrate and consists of unbound Ti, Ti2Ni, TiNi, Cu0.81Ni0.19 and CuTi. The results indicate that the treated samples exhibit excellent antibacterial properties within 12 h. The wear resistance of titanium alloy is also improved.