Micro/nano multi-level structure tantalum coating was prepared on medical titanium substrate surface using plasma spraying technique. The surface morphology, chemical composition and phase composition were analyzed by SEM, EDS and XRD, and the adhesion between substrate and coating was investigated by tensile method. The surface adsorption amount of protein of micro/nano multi-level tantalum coating and titanium substrate was compared by using BSA. The result showed that when the titanium-based tantalum coating was prepared under the spray distance was 110 mm and spray power was 30 kW, typical multi-level structural features can be seen, combined with good binding force and high Ta phase content; protein adsorption test showed that titanium-based tantalum coatings have good protein adsorption capacity, whose adsorption amount were higher than control samples.